Figure 3.

NMR spectra of CAC1 and CA-C. (A) Up-field region of the spectrum of CAC1 at 10 μM. (B) Up-field region of the spectrum obtained by addition of the spectra of CAC1 and CA-C. (C) Up-field region of the spectrum of the heterocomplex formed between CA-C and CAC1 at the same concentrations (10 μM of each macromolecule). (D) Up-field region of CA-C at 10 μM. The conditions were phosphate buffer (pH 7.3), 25 mM, at 298 K in ²H₂O (no corrections were done for isotope effects). The sharp signal at the right side observed in all spectra, except in that of CAC1, is the NMR internal reference (TSP). The intensity of the signals for each NMR spectrum is arbitrary.