Table 1.

Winter flounder HPLC6 and its mutants

Code	Sequence					Hysteres, % to WF AFP at 2 mM	α-Helicity,%	Reference
	1	2	13	24	35
HPLC6	D	TASDAAAAAAL	TAANAKAAAEL	TAANAAAAAAA	TAR	100	98	Loewen et al. (1999)
S23	D	TASDAAAAAAL	NAATAKAAAEL	TAAAAANAAAA	TAR	17	95.3	Wen and Laursen (1993)
TSST	D	TASDAAAAAAL	SAANAKAAAEL	SAANAAAAAAA	TAR	0.11	100	Haymet et al. (1998)
SSSS	D	SASDAAAAAAL	SAANAKAAAEL	SAANAAAAAAA	SAR	0	100	Haymet et al. (1998)
STSS	D	SASDAAAAAAL	TAANAKAAAEL	SAANAAAAAAA	SAR	0	105	Zhang and Laursen (1998)
SSSS2KE	D	SASDAKAAAEL	SAANAKAAAEL	SAANAKAAAEA	SAR	0	100	Haymet et al. (1998)
TVVT	D	TASDAAAAAAL	VAANAKAAAET	VAANAAAAAAA	TAR	85	100	Chao et al. (1997)
VVVV2KE	D	VASDAKAAAEL	VAANAKAAAEL	VAANAKAAAEA	VAR	100	100	Haymet et al. (1998)
AAAA2KE	D	AASDAKAAAEL	AAANAKAAAEL	AAANAKAAAEA	AAR	17	100	Haymet et al. (1998)
S21	D	TASADAAAAAN	LAANAKAAAEL	TAAAAANAAAA	TAR	0	106.2	Wen and Laursen (1993)
S22	D	TASDAAAAAAN	LAATAKAAAEL	TAAAAANAAAA	TAR	30	96.4	Wen and Laursen (1993)
S41	D	TASDAALAAAA	TAANKLAAAEA	TAANAAAAAAA	TAR	0	103	Wen and Laursen (1993)
S42	D	TASDAAAAAAA	TAANLKAAAEA	TAANAAAAAAA	TAR	0	102	Wen and Laursen (1993)
A17L	D	TASDAAAAAAL	TAANLKAAAEL	TAANAAAAAAA	TAR	0		Baardsnes et al. (1999)
A19L	D	TASDAAAAAAL	TAANAKLAAEL	TAANAAAAAAA	TAR	80		Baardsnes et al. (1999)
A20L	D	TASDAAAAAAL	TAANAKALAEL	TAANAAAAAAA	TAR	100		Baardsnes et al. (1999)
A21L	D	TASDAAAAAAL	TAANAKAALEL	TAANAAAAAAA	TAR	10		Baardsnes et al. (1999)