Table 1.
Linker sequences of functional single-chain penicillin acylases
| Enzyme | Specific activitya % |
| WTb | 100 |
| NEGMb | 22 |
| PGLR | 15 |
| GGEA | 14 |
| DPAGb | 35 |
| GGLK | 8 |
| EPQR | 5 |
| GGG | 18 |
| RGAG | 27 |
| PGVG | 18 |
| GDAL | 16 |
| QGAG | 13 |
| GGSG | 7 |
| RAAV | 3 |
| AESSc | 29 |
| GARDc | 39 |
a Measured using an XL1-Blue total crude soluble-protein extract by the PDAB method. The PGA wild-type value (28.9 μg per min of 6-APA formed using 100 μg of soluble protein) was taken as 100%.
b Those clones were expressed in both pT4Bla and pThioC expression vectors.
c Functional clones obtained from the library constructed with the pThioC expression vector.