. 2004 Jun;13(6):1677–1683. doi: 10.1110/ps.03436604

Table 2.

Active-site titration experiments for some variants and the heterodimeric protein

	k_cat^a	Active site concentration μM PMSF
Enzyme	sec⁻¹	Experiment 1	Experiment 2
WT	25.1 ± 1.4	0.28 (0.55)^b	0.56 (1.1)
NEGM	36.8 ± 2.7	0.13 (0.14)	0.22 (0.28)
DPAG	15.5 ± 0.8	0.20 (0.21)	0.54 (0.62)

^a Determined by PMSF inhibition studies (Fig. 3 ▶).

^b The data in parentheses indicate the protein molar concentration (in μM) used for the inhibition experiments. The different inhibition experiments consisted in modifying the amount of initial protein before measuring the active-site molar concentration.