Figure 2.

In vitro ribosome inactivation by E3 rRNase mutants. Mutant rRNase domains were assessed for the ability to inactivate ribosomes in vitro through a coupled transcription-translation assay at an rRNase concentration of 10 nM. Ribosome function was monitored through the production of a firefly luciferase reporter protein. Data are presented as the translational activity of the in vitro system after colicin E3 treatment normalized to the no E3 rRNase control. Error bars represent the standard deviation from two independent experiments (see Materials and Methods).