Table 1.

Equilibrium dissociation constants, Kd, measured at increasing SDS concentrations

SDS (μM)	Kd (μM)	Kdapp* (μM)
0	3.1 ± 0.3	8.2
50	6.3 ± 0.6	17.8
100	16.2 ± 1.6	n.d.
250	n.d.
500	n.d.
1000	n.d.

Equilibrium dissociation constants, K_d, obtained by fluorescence titrations of β-DG(654–750) with a synthetic peptide α-DG(549–567) (central column, standard errors are reported) and by solid-phase binding assays of biotinylated β-DG(654–750) with Trx-α-DG(485–600) (right column), carried out in the presence of increasing SDS concentrations. (*) Apparent equilibrium dissociation constants. K_d values represent an average of three independent experiments. (n.d.) Not determined.