Figure 1.
The effect of denaturant on effective ΔG of multimeric proteins. n represents the oligomeric state of each protein. The m/n values and Cm of all the proteins were set to be 2 kcal/(mole•M) and 3 M, respectively, for comparison. ΔG°(H2O) was determined with equation 14 by using these parameters. The fraction of unfolded protein, fD, was determined from ΔG° by solving equation 4 numerically. ΔGeff was calculated with this fD by equation 5. Total protein concentration was set to be 2 μM in monomer units. However, the result is indifferent to total protein concentration, because Cm was fixed.