Table 2.

Comparison of m_effat C_mwith m-values determined with a two-state monomer model

Oligomeric state	m-value used for generating simulation data	meff at Cm	m-value from curve fitting with a monomer model
1	−2.0	−2.0	−2.0 ± 0.0
2	−4.0	−2.7	−2.7 ± 0.1
3	−6.0	−3.0	−3.1 ± 0.1
4	−8.0	−3.2	−3.3 ± 0.1
6	−12.0	−3.4	−3.5 ± 0.2

The fractions of unfolded protein, f_D, were calculated by assuming m/n = −2 kcal/(mole • M) and C_m = 3 M for imaginary n-mer proteins (Fig. 1 ▶). These f_D values were fit with a two-state monomer model to determine m-values. The m_eff at C_m were also calculated with equation 13 from the m-values used to calculate f_D. The unit of m-values is kcal/(mole • M).