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Circular dichroism of W9F αA- and γC-crystallin mixtures in near-UV region (upper panel) and far-UV region (lower panel). (A) W9FαA- and γC-crystallin; (B) W9F αA- and T5P γC-crystallin; (C) W9F αA-crystallin alone. Curve 1, controls; curve 2, preheated samples at 65°C for 1 h. Protein concentrations were 0.25 and 0.15 mg/mL for αA- and γC-crystallin, respectively. Cell path length was 10 mm and 1 mm for the measurements at near- and far-UV regions, respectively.
