Table 2.

Disulfide analysis of CRF_2(a) receptor NT determined by MALDI-MS of cleaved fragments

Fragment	Cysteine	Cysteine pattern	Calc. [M+H]+	Found [M+H]+
N-(His)6-CRF2(a) receptor NT, hydroxylamine, GluC
35-61	Cys40-Cys50	C2-C3	2869.30	2869.40
62-66+87-103	Cys64-Cys98	C4-C6	2546.20	2548.81
CRF2(a)-C-(His)6 receptor NT, chymotrypsin, cyanylation
40-44+81-89	Cys40-Cys83	C2-C5	1655.71	1655.74
38-59+81-84a	Cys40,50-Cys83	C2,3-C5	2868.42	2868.21
45-68+96-102a	Cys50,64-Cys98	C3,4-C6	3486.93	3486.90
CRF2(a)-C-(His)6 receptor NT, chymotrypsin, alkylation
39-43+80-88	Cys40-Cys83	C2-C5	1655.70	1655.50
59-67+95-108	C64-Cys98	C4-C6	2868.31	2868.30
39-58+80-88b	Cys40,50-Cys83	C2,3-C5	3486.80	3485.48

^a Cyanylation adduct.

^b Glutathione adduct.