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. 2004 Sep;13(9):2470–2475. doi: 10.1110/ps.04835904

Table 3.

Results of ligand binding studies by SPA

CRF2(a)-C-(His)6 receptor NT N-(His)6-CRF2(a) receptor NT
Ligand EC50 [nM] EC50 [nM]
Rat urocortin 1 (8–40) 45.6 ± 4.9 71.0 ± 10.1
Astressin 6.8 ± 1.5 4.6 ± 0.5

The data are means of at least three different experiments performed in triplicates using [125I]Tyr0-urocortin as radioligand.