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Electrophoretic profiles of cytochrome b5 isoforms expressed in E. coli strains engineered with peptide appendages interlinking signal sequence and the amino terminus of passenger cytochrome. (A) Periplasmic proteins (•) resolved in non-denaturing polyacrylamide [15% (w/v)] gel; (B) the corresponding precursor pools in the isolated inner membranes separated in polyacrylamide gel (17%) containing 0.1% SDS. Vertical arrows mark protein profiles of the control E. coli pAF cell line.
