Figure 1.

Reactivation of dr-API in presence and absence of PDI. (A) Reactivation of 0.5 μM dr-API in absence (circles) and presence (down-pointing triangles) of 5 μM PDI and 2 μM dr-API in absence (up-pointing triangles) and presence (squares) of 20 μM PDI was initiated in redox buffer as described in Materials and Methods, and the recovery of anti-proteolytic activity was determined by the caseinolytic assay. (B) The figure shows the initial time points (up to 20 min) of the curves as in A. The curves represent the best fit of the reactivation data that are expressed as mean ± SD (n = 3 to 5).