Figure 4.

Influence of PDI on aggregation of dr-API during refolding. (A) The aggregation of 2 μM dr-API in the presence of varying dr-API/PDI ratios during refolding was monitored by light scattering at 385 nm. The molar ratios of dr-API to PDI were as follows: (a) 1:0; (b) 1:1; (c) 1:5; and (d) 1:10. The data are expressed as mean ± SD (n = 3 to 5). (B) The time-dependent suppression of aggregation of 2 μM dr-API in presence of varying amounts of PDI was monitored in redox buffer for 30 min at 25°C. The molar ratios of dr-API to PDI were 1:0 (circles), 1:1 (down-pointing triangles), 1:5 (up-pointing triangles), and 1:10 (squares).