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Effect of delayed addition of PDI on reactivation of dr-API in redox buffer. Refolding of dr-API 0.05 μM (squares), 0.5 μM (circles), and 2 μM (up-pointing triangles) was initiated in redox buffer followed by an addition of 10-fold molar PDI after 2 h. As a control, 0.05 μM dr-API was refolded in the redox buffer without addition of PDI (down-pointing triangles). The arrow indicates the time of addition of PDI. The data are expressed as mean ± SD (n = 3 to 5).
