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. Author manuscript; available in PMC: 2009 Mar 15.
Published in final edited form as: Dev Biol. 2008 Jan 16;315(2):459–473. doi: 10.1016/j.ydbio.2008.01.005

Fig. 9.

Fig. 9

Hedgehog signaling is significantly reduced in Runx2 null skin at E18.5. (A) By in situ hybridization the patterns of expression of Sonic hedgehog and downstream mediators are similar to those previously reported. There are no differences in apparent expression levels between wild-type and null animals. Note that the expression of Sonic hedgehog is restricted to the proximal (proliferating end) of the more mature follicles and tends to predominate on the superficial side. This asymmetrical pattern is strikingly similar to the epithelial expression of Runx2 (see text and Fig.1D). (B) Sonic hedgehog mRNA is reduced by 34%, Patched1 by 67% and Gli1 by 82% utilizing real-time PCR. Note that the bars represent relative expression levels with wild-type values set at 1. Wild-type (WT), Sonic hedgehog (Shh), Patched1 (Ptc1). *p=0.06, **p=0.04, ***p=0.001. Error bars indicate ±1 standard deviation.