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The steady-state parameters for the hydrolysis of the sensitive trypsin substrate Tos-Gly-Pro-Arg-pNA, in 100 mM Tris, 20 mM CaCl2(pH 8.3) for AST, CST, and BT
| AST | CST | BT | |
| kcat (S−1) | 30.4 | 87.5 | 88.0 |
| Km (M) | 8.96 * 10−7 | 2.61 * 10−6 | 5.19 * 10−6 |
| kcat/Km (S−1M−1) | 3.39 * 107 | 3.35 * 107 | 1.70 * 107 |
