Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Representative calorimetric titration of SecB into SecA. SecA at 7 μM dimer was held in the cell at 8°C. SecB, held in the syringe at 190 μM tetramer, was added in a sequence of 21 injections, each of 10 μL, spaced at 8-min intervals. (Top) Raw data. (Bottom) Integrated area of heat as a function of the mole ratio of the reactants. The points are experimental data, and the solid line is the calculated best fit using a least-squares deconvolution algorithm.
