Table 1.
Sequences of RT-PCR clones derived from 5S RNA
| Sequence | No. of clones
|
|
|---|---|---|
| Wild type | RNase T−PH−D−BN− | |
| GGAACTGCCAGGCAT (mature) | 8 | 0 |
| GGAACTGCCAGGCA | 1 | 0 |
| GGAACTGCCAGGA | 1 | 0 |
| GGAACTGCCAGGCATA | 1 | 1 |
| GGAACTGCCAGGCATC | 1 | 4 |
| GGAACTGCCAGGCATCA | 0 | 2 |
| GGAACTGCCAGGCATCAA | 0 | 2 |
| GGAACTGCCAGGCATCAAA | 0 | 1 |
| GGAACTGCCAGGCATCAAAA | 0 | 2 |
| GGAACTGCCAGGCATCAAAAA | 0 | 1 |
| GGAACTGCCAGGCATCAAAAAAA | 0 | 2 |
Sequences around the 3′ end of 5S RNA are shown, and are written 5′ to 3′. Plain text indicates sequences present in mature 5S RNA. Underlined nucleotides represent precursor residues present in the primary transcript. Residues in boldface type are not encoded by 5S RNA genes. Italicized A residues may either be encoded or added posttranscriptionally; data presented in Table 3 and Fig. 1 support posttranscriptional addition.