Table 3.
Summary of sequences of RT-PCR clones derived from stable RNA species from wild-type and exoribonuclease-deficient cells
| RNA species | Strain | Shorter than mature | Mature | Mature + adenylates | Precursor | Precursor + adenylates |
|---|---|---|---|---|---|---|
| 5S | Wild-type | 2 {1}* | 8 | 1 {1} | 1 | 0 |
| T−PH−D−BN− | 0 | 0 | 1 {1} | 4 | 10 {1–7} | |
| T−PH−D−BN−PAP− | 3 | 1 | 0 | 6 | 0 | |
| M1 | Wild-type | 1 | 5 | 0 | 1 | 0 |
| T−PH−D−BN− | 0 | 0 | 0 | 0 | 12 {1–3} | |
| tRNATyr | Wild-type | 0 | 5 | 0 | 0 | 0 |
| T−PH−D−BN−/tyr1 | 1 | 1 | 1 {5} | 8 | 19 {1–3} | |
| T−PH−D−BN−/tyr2 | 0 | 8 | 10 {3–5} | 0 | 0 | |
| tmRNA | Wild-type | 0 | 5 | 0 | 0 | 0 |
| T−PH−D−BN− | 0 | 10 | 6 {3–4} | 0 | 0 | |
| 4.5S | Wild-type | 0 | 4 | 0 | 0 | 0 |
| T−PH−D−BN− | 1 {5} | 1 | 0 | 2 | 13 {3–4} | |
| T−PH−D−BN−PAP− | 0 | 4 | 0 | 5 | 0 | |
| 6S | Wild-type | 1 | 5 | 0 | 0 | 0 |
| T−PH−D−BN− | 0 | 4 | 1 {4} | 9 | 5 {1–4} | |
| 23S | Wild-type | 0 | 7 | 0 | 0 | 0 |
| T−PH−D−BN− | 2 | 1 | 0 | 10 (5) | 9 {1–5} | |
| 16S | Wild-type | 2 | 5 | 0 | 1 (1) | 0 |
| T−PH−D−BN− | 1 | 9 | 0 | 3 (3) | 1 {4} |
The values presented are the number of RT-PCR clones recovered derived from RNA with the indicated 3′ terminal sequence. Values in parentheses ( ) indicate the number of clones having a single 3′ terminal adenylate residue that either can be encoded or added by polyadenylation. Values in brackets { } are the lengths of adenylate tails shown to be added posttranscriptionally.
*
One of the two clones for 5S RNA has a nonencoded adenylate residue at its 3′ end.