Figure 3.

Inhibition of cytochrome c binding to anionic membranes by FABP. (A) Increasing concentrations of cytochrome c were incubated with 12.5 μMSUV (EPC/EPE/CL/DPE, 64:10:25:1). The quenching of dansyl fluorescence is expressed relative to the fluorescence intensity of vesicles without cytochrome c. (B) 12.5 μM SUV containing 1% DPE were incubated with increasing concentrations of IFABP (•) or FABP-HL (▾) for 5 min, and 0.75 μM cytochrome c was then added as described in Materials and Methods. Results are expressed as the percent relative fluorescence intensity, where 100% represents the relative fluorescence intensity of SUV incubated in the presence of cytochrome c, but without FABP. Results are the average of three experiments ± SD.