Figure 4.

RNase H Digestion of RNA. (A) The diagram shows the products formed after binding the primer E4r to RNA and digesting with RNase H. An analogous diagram can be formulated with the other three primers used. (B) Total RNA from liver of adult rat was bound in different tubes with primers E4r (exon 4), E4r-2 (exon 4), E5r (exon 5), and E7r (exon 7) and then digested with RNase H and analyzed on a 1.6% agarose gel with formaldehyde as described in Materials and Methods (lanes 2–5). Lane 1 denotes control nondigested RNA. The filter containing the transferred RNA was hybridized to a ³²P-labeled cDNA probe corresponding to positions 31–1,700 bp in the cDNA sequence from COT (14). RNA molecular size markers (M) are indicated to the right.