Exon 1 |
E1f |
Sense, 22-mer |
5′GAG TGC AGA GAG CCA AGC CGG G3′ |
RT-PCR |
Exon 2 |
E2f |
Sense, 25-mer |
5′TTT TCT TAC TGT GAC TAT ACC ATG G3′ |
RT-PCR and trans-splicing |
Exon 2 |
E2r |
Antisense, 22-mer |
5′GGA ATG TTC GTT CTT CAA TTG A3′ |
RT-PCR |
Exon 3 |
E3r |
Antisense, 20-mer |
5′TGA TGC AAT GTC TTG CCA AC3′ |
RT-PCR |
Exon 4 |
E4r |
Antisense, 20-mer |
5′GGC GAC ATT GAG CCA CCA CT3′ |
RT-PCR and RNase H protection |
Exon 5 |
E5r |
Antisense, 18-mer |
5′GGT TCA TGT CTA GAG GAG3′ |
RT-PCR and RNase H protection |
Exon 7 |
E7r |
Antisense, 21-mer |
5′GGC CCA ACA GGT TCA TTC CAG3′ |
RT-PCR and RNase H protection |
Exon 4 |
E4r-2 |
Antisense, 21-mer |
5′CTT CTT AGC AGC TGC CAG TAG3′ |
RNase H protection |
Intron 2 |
I2r |
Antisense, 25-mer |
5′CTA CAC TAG AAG ATT ATG AAC ATA C3′ |
Trans-splicing |
Intron 1 |
I1f |
Sense, 20-mer |
5′CAT GGG GTG CTA AAT CCA CA3′ |
Trans-splicing |
Exon 2 |
E2r-2 |
Antisense, 22-mer |
5′CTG ACT CAA GGT ACT TCT TCA G3′ |
Trans-splicing |