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. 1998 Oct 13;95(21):12185–12190. doi: 10.1073/pnas.95.21.12185

Table 1.

Oligonucleotides used in this study

Position Primers Orientation Sequence Expt(s)
Exon 1 E1f Sense, 22-mer 5′GAG TGC AGA GAG CCA AGC CGG G3′ RT-PCR
Exon 2 E2f Sense, 25-mer 5′TTT TCT TAC TGT GAC TAT ACC ATG G3′ RT-PCR and trans-splicing
Exon 2 E2r Antisense, 22-mer 5′GGA ATG TTC GTT CTT CAA TTG A3′ RT-PCR
Exon 3 E3r Antisense, 20-mer 5′TGA TGC AAT GTC TTG CCA AC3′ RT-PCR
Exon 4 E4r Antisense, 20-mer 5′GGC GAC ATT GAG CCA CCA CT3′ RT-PCR and RNase H protection
Exon 5 E5r Antisense, 18-mer 5′GGT TCA TGT CTA GAG GAG3′ RT-PCR and RNase H protection
Exon 7 E7r Antisense, 21-mer 5′GGC CCA ACA GGT TCA TTC CAG3′ RT-PCR and RNase H protection
Exon 4 E4r-2 Antisense, 21-mer 5′CTT CTT AGC AGC TGC CAG TAG3′ RNase H protection
Intron 2 I2r Antisense, 25-mer 5′CTA CAC TAG AAG ATT ATG AAC ATA C3′ Trans-splicing
Intron 1 I1f Sense, 20-mer 5′CAT GGG GTG CTA AAT CCA CA3′ Trans-splicing
Exon 2 E2r-2 Antisense, 22-mer 5′CTG ACT CAA GGT ACT TCT TCA G3′ Trans-splicing