Skip to main content
. 1998 Oct 13;95(21):12202–12207. doi: 10.1073/pnas.95.21.12202

Figure 2.

Figure 2

Rsk-2 is necessary for CREB phosphorylation upon activation of EGF signaling pathway. Normal (RSK2+) and CLS (RSK2−) cells were starved 24 hr in the absence of serum before harvesting. Cell extracts were quantified by Coomassie blue staining. Lanes: 2, forskolin was utilized at a concentration of 10 μg/ml for 30-min stimulation; 3, cells were irradiated for 10 sec and harvested 30 min after exposure to UV (as in ref. 20); 4, fetal calf serum was added at 20% final concentration for 10 min; 5 and 6, cells were exposed to 30 ng/ml of EGF for 5 and 10 min (lanes 5 and 6, respectively). CREB phosphorylation levels in Rsk-2− cells upon EGF induction are about 5% of the forskolin-induced level in the same cells and 10% of the corresponding EGF induction in Rsk-2+ cells, after adjusting with the basal levels.