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. 1998 Oct 13;95(21):12202–12207. doi: 10.1073/pnas.95.21.12202

Figure 3.

Figure 3

MAPK response is normal in cells from CLS patients. Normal (RSK2+) and CLS (RSK2−) cells were starved 24 hr in the absence of serum before treatment and then harvested. An antibody recognizing the phosphorylated MAPK was used (see Materials and Methods). Lanes: 2, forskolin was added at the concentration of 10 μg/ml for 10 min; 3, cells were exposed for 10 sec to UV rays and harvested 30 min after irradiation (20); 4, samples were stimulated in the presence of 20% fetal calf serum for 10 min; 5 and 6, cells were treated with 30 ng/ml of EGF for the indicated times. Total levels of MAPK are uniform in all samples.