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Rsk-2 is not necessary for TCF/Elk-1 phosphorylation. Normal (RSK2+) and CLS (RSK2−) fibroblasts were deprived of serum for 24 hr before treatment. Lanes: 2, 10 μg/ml of forskolin were used for 30-min stimulation; 3, cells were UV-irradiated for 10 sec and harvested 20 min later (19); 4, fetal calf serum was added at a 20% concentration; 5, EGF was used at the concentration of 30 ng/ml for 10-min stimulation.
