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. 1998 Oct 13;95(21):12202–12207. doi: 10.1073/pnas.95.21.12202

Figure 7.

Figure 7

The CRE is sufficient to mediate induction of c-fos expression by Rsk-2. (A) Schematic representation of FC4 and FC8 reporter plasmids (35); binding sites for Elk-1/SRF (SRE) and CREB (CRE) are indicated. (B) COS cells were transfected with 0.5 μg of FC4 and FC8 reporter plasmids in combination with 0.2 μg of CREB expression vector and 2 μg of Rsk-2 expression vector when indicated. Cells were harvested 36 hr after addition of calcium phosphate precipitate. Rsk-2-mediated activation is 7.5 (±0.5)-fold for FC4 and 4.5 (±0.5)-fold for FC8, as established by photometric scanning of the autoradiograms. The results presented are representative of three independent experiments.