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Substrates for E. coli MetRS. (Top) Sequences of E. coli elongator and initiator tRNAs. The base pairs at the first position of the acceptor stems are shaded. (Middle) Microhelix substrates that were assayed. Wild-type microhelixfMet and microhelixMet substrates and variants of microhelixMet are shown, with substitutions of the first base pair being shaded. (Bottom) DuplexMet substrates, with base substitutions shaded.
