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Enhancement of aminoacylation by terminal nucleotide deletion. (Upper) Acid gel analysis of microhelix aminoacylation showing the enhanced aminoacylation of the Δ1⋅C72 microhelixMet substrate with [35S]methionine after a 15-min reaction. (Lower) Time course of aminoacylation of microhelixMet and Δ1⋅C72 microhelixMet. The enhanced aminoacylation conferred by the Δ1⋅C72 substitution is also seen with the duplexMet substrate (Inset).
