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Enhancement of minihelixIle aminoacylation by deletion of nucleotide A1. (Upper) MinihelixIle recapitulates the acceptor stem and TΨC loop of tRNAIle. Δ1⋅U72 minihelixIle is lacking the 5′ terminal adenosine. (Lower) Time course of aminoacylation of minhelixIle and Δ1⋅U72 minihelixIle.
