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Detection of 3H-DFP-labeled plasma proteins after treatment with Micrococcus lysodeikticus. Larval plasma samples (14 μl) were preincubated with M. lysodeikticus (1 μl, 1 μg/μl) and 0.1 M glutathione (2 μl) for 0, 30, 60, and 120 min, then allowed to react with 3H-DFP for 30 min. After separation by SDS/PAGE, labeled proteins were detected by fluorography. Lane M contains 14C-protein molecular mass markers.
