Figure 5.

Sequence alignment of M. sexta PAP with other arthropod serine proteinases that have a clip domain. The catalytic triad residues are boxed. Residues conserved in all of the sequences are labeled with an asterisk. A vertical double line marks the putative activation cleavage sites. Conserved cysteines, whose disulfide bonding pattern has been determined in PCE (31), are connected with single lines. A pair of cysteines present only in PAP and easter is labeled with +. Anopheles gambiae SP14D (AF007166), Drosophila easter (P13582), M. sexta hemocyte proteinase 1 (HP1) (AF017663), and horseshoe crab proclotting enzyme (PCE) (P21902).