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C126, R132, H125, T133, and D66 are important for mouse RTP activity. γ-32P-labeled RNA was incubated with 100 ng of the indicated purified proteins as described in Materials and Methods. Released 32Pi was quantitated by PhosphorImager. Values are expressed relative to 1.0 for GST-MCE (1–237). Variation among several experiments was ±0.1.
