Table 1.

m/z of peptide/oligosaccharide complexes used in this study

Substrates (1–4, 14) and products (5–13, 15–25)		Underivatized	Derivatized
Monosaccharides
M24	HNAc,6S	(4,742.19)
Disaccharides
Di6	ΔU2S–HNS,6S	4,804.23	4,859.25
Di15	I2S–HNS,6S	4,822.26
Di17	ΔU2S–Man6S	(4,709.15)
Di18	ΔU–HNS,6S	(4,724.17)
Di23	ΔU2S–HNS,6S,OMe	4,822.26
Trisaccharides
Tr22	HNAc,6S–G–HNS,6S,3S	5,143.49
Tetrasaccharides
T5	ΔU2S–HNS,6S–I2S–HNS,6S	5,381.72	5,437.79
T7	ΔU2S–HNS,6S–I2S–Man6S	5,286.63
T9	ΔU2S–HNS,6S–G–HNS,6S	5,301.65
T16	I2S–HNS,6S–I2S–HNS,6S	(5,399.74)
T19	ΔU2S–HNS,6S–I–HNAc,6S	5,263.63
T25	ΔU–HNS,6S,3S–I2S–HNS,6S,OMe	(5,410.72)
Pentasaccharides
P14	HNAc,6S–G–HNS,6S,3S–I2S–HNS,6S,OMe	5,734.99
Hexasaccharides
H1	I2S–HNS,6S–I2S–HNS,6S–I2S–Man6S	5,882.13
H8	ΔU2S–HNS,6S–[I2S–HNS,6S]2	5,959.20	6,015.27
H10	ΔU2S–HNS,6S–I2s–HNS,6S–G–HNS,6S	5,879.13
H12	ΔU2S–HNS,6S–I–HNAc,6S–HNS,6S,3S	5,841.11
H20	ΔU–HNS,6S,3S–I2S–HNS,6S–I2S–HNS,6S	5,959.20
Octasaccharides
O2	ΔU2S–HNS,6S–I2S–HNS,6S–I2S–HNS,6S–G–HNS,6S	6,456.61
O11	ΔU2S–HNS,6S–[I2S–HNS,6S]3	6,536.68
O13	ΔU2S–HNS,6S–I–HNAc,6S–G–HNS,6S,3S–I2S–HNS,6S	6,536.68	6,592.75
O21	ΔU–HNAc,6S–G–HNS,6S,3S–I2S–HNS,6S–I2S–HNS,6S	6,536.69
Decasaccharides
D3	ΔU2S–HNS,6S–[I2S–HNS,6S]4	(6,996.07)	7,052.14
D4	ΔU2S–HNS,6S–I–HNAc,6S–G–HNS,6S,3S–[I2S–HNS,6S]2	(7,114.16)

2S, 3S, and 6S, 2-O, 3-O, or 6-O sulfation, (respectively); NS and NAc, N-sulfation and N-acetylation of the glucosamine. 

^*Saccharides not observed are in parentheses. Oligosaccharides are abbreviated as follows: I, α-l-iduronic acid; G, β-d-glucuronic acid; ΔU, I or G with an unsaturated C4–C5 bond; H, α-d-2-deoxy,2-aminoglucose; and Man, anhydromannitol.