Figure 7.

Cell-free translation of S15-luciferase fusion RNAs. (A) Schematic representation of the reporter plasmids indicating the wild-type (S15-W) and mutated S15 sequences (S15-M1 to S15-M4). The amino acid sequences encoded by S15-W and mutations S15-M1 to S15-M4 are listed below the nucleotide sequences. The S15 and luciferase sequences are shown as open bars, and the T7 promoter is indicated as an arrow. (B) Effect of complementarity between S15 mRNA and 18S rRNA on translation of S15-luciferase fusion mRNAs. Luciferase activity is expressed as relative light units (RLU). The luciferase activity for each construct was calculated from four independent experiments, and the average was plotted ± SEM.