Table 1.
Description of RNA probes
| Probe | Sequence | Complementarity to mouse rRNA | Location in mRNA | Length | Accession code |
|---|---|---|---|---|---|
| 18S-a | 18S rRNA | 100% in 86 nt of 18S rRNA (nt 227–312) | — | 86 nt | X00686 |
| 18S-b | 18S rRNA | 100% in 139 nt of 18S rRNA (nt 645–783) | — | 139 nt | X00686 |
| 18S-c | 18S rRNA | 100% in 15 nt of 18S rRNA (nt 916–930) | — | 15 nt | X00686 |
| 18S-d | 18S rRNA | 100% in 57 nt of 18S rRNA (nt 1294–1351) | — | 58 nt | X00686 |
| 18S-e(α) | 18S rRNA | 100% in 54 nt of 18S rRNA (nt 1816–1869) | — | 54 nt | X00686 |
| 28S-G | 28S rRNA | 100% in 59 nt of 28S rRNA (nt 1767–1825) | — | 59 nt | X00525 |
| S15 | Ribosomal protein | 91% in 22 nt of 18S rRNA (nt 1847–1866) | C, nt 62–83 | 56 nt | M33330 |
| HSP68 | Heat shock protein (HSP68) | 88% in 16 nt of 18S rRNA (nt 274–289) | C, nt 147–162 | 30 nt | M12573 |
| CAL | Calcineurin catalytic subunit | 72% in 36 nt of 18S rRNA (nt 251–286) | 5′, nt 176–211 | 36 nt | M81475 |
| EPO | Erythropoietin | 100% in 20 nt of 18S rRNA (nt 257–276) | 5′, nt 392–411 | 29 nt | M12930 |
| TROPO | Nonmuscle tropomyosin 5 | 79% in 14 nt of 18S rRNA (nt 688–701) | 3′, nt 1609–1622 | 27 nt | X53753 |
| HMGI | High mobility group I protein | 77% in 34 nt of 18S rRNA (nt 729–762) | 3′, nt 530–563 | 34 nt | X58380 |
| AC | Adenyl cyclase type VI | 90% in 20 nt of 18S rRNA (nt 746–765) | 3′, nt 3769–3788 | 44 nt | M93422 |
| UBF | Transcription factor | 92% in 13 nt of 18S rRNA (nt 918–930) | 3′, nt 2867–2879 | 29 nt | X60831 |
| DMT | DNA (cytosine-5)-methyltransferase | 100% in 11 nt of 18S rRNA (nt 918–928) | C, nt 328–338 | 29 nt | X14805 |
| GTX | Homeobox protein | 79% in 14 nt of 18S rRNA (nt 1118–1131) | 5′, nt 133–169 | 42 nt | L08074 |
| IL-2 | Interleukin 2 receptor gamma chain | 79% in 24 nt of 18S rRNA (nt 1311–1334) | 3′, nt 1484–1507 | 37 nt | L20048 |
| GTBP | GTP binding protein | 84% in 25 nt of 18S rRNA (nt 1481–1505) | 5′, nt 36–60 | 27 nt | U15636 |
| CALM | Calmodulin kinase II | 74% in 34 nt of 18S rRNA (nt 1549–1582) | C, nt 1481–1514 | 34 nt | X14836 |
| mRNA-1c | See ref. 19 | — | — | 56 nt | — |
The 18S and 28S probes listed in the first six rows are based on rRNA sequences. Probes 18S-a, -b, -c, -d, and 28S-G were synthesized as both sense (s) and antisense (α) molecules. The 13 mRNA probes, including S15, are listed below the rRNA probes. C, coding region; 5′, 5′UTR; 3′, 3′ UTR.