Abstract
Ninety-six patients with chronic hepatitis C were studied. A second-generation recombinant immunoblot assay detected anti-NS4 antibodies significantly more often in patients infected by hepatitis C virus genotype 1 than in patients infected by other types. By a third-generation recombinant immunoblot assay, the prevalences of the four antibodies measured did not differ according to the hepatitis C virus genotype.
Full Text
The Full Text of this article is available as a PDF (154.5 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Alter H. J. New kit on the block: evaluation of second-generation assays for detection of antibody to the hepatitis C virus. Hepatology. 1992 Feb;15(2):350–353. doi: 10.1002/hep.1840150228. [DOI] [PubMed] [Google Scholar]
- Brown J., Dourakis S., Karayiannis P., Goldin R., Chiba J., Ohba H., Miyamura T., Thomas H. C. Seroprevalence of hepatitis C virus nucleocapsid antibodies in patients with cryptogenic chronic liver disease. Hepatology. 1992 Feb;15(2):175–179. doi: 10.1002/hep.1840150202. [DOI] [PubMed] [Google Scholar]
- Dusheiko G., Schmilovitz-Weiss H., Brown D., McOmish F., Yap P. L., Sherlock S., McIntyre N., Simmonds P. Hepatitis C virus genotypes: an investigation of type-specific differences in geographic origin and disease. Hepatology. 1994 Jan;19(1):13–18. [PubMed] [Google Scholar]
- Jeffers L. J., Hasan F., De Medina M., Reddy R., Parker T., Silva M., Mendez L., Schiff E. R., Manns M., Houghton M. Prevalence of antibodies to hepatitis C virus among patients with cryptogenic chronic hepatitis and cirrhosis. Hepatology. 1992 Feb;15(2):187–190. doi: 10.1002/hep.1840150204. [DOI] [PubMed] [Google Scholar]
- Kuo G., Choo Q. L., Alter H. J., Gitnick G. L., Redeker A. G., Purcell R. H., Miyamura T., Dienstag J. L., Alter M. J., Stevens C. E. An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science. 1989 Apr 21;244(4902):362–364. doi: 10.1126/science.2496467. [DOI] [PubMed] [Google Scholar]
- Marcellin P., Martinot-Peignoux M., Boyer N., Pouteau M., Aumont P., Erlinger S., Benhamou J. P. Second generation (RIBA) test in diagnosis of chronic hepatitis C. Lancet. 1991 Mar 2;337(8740):551–552. doi: 10.1016/0140-6736(91)91335-r. [DOI] [PubMed] [Google Scholar]
- McOmish F., Yap P. L., Dow B. C., Follett E. A., Seed C., Keller A. J., Cobain T. J., Krusius T., Kolho E., Naukkarinen R. Geographical distribution of hepatitis C virus genotypes in blood donors: an international collaborative survey. J Clin Microbiol. 1994 Apr;32(4):884–892. doi: 10.1128/jcm.32.4.884-892.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nakatsuji Y., Matsumoto A., Tanaka E., Ogata H., Kiyosawa K. Detection of chronic hepatitis C virus infection by four diagnostic systems: first-generation and second-generation enzyme-linked immunosorbent assay, second-generation recombinant immunoblot assay and nested polymerase chain reaction analysis. Hepatology. 1992 Aug;16(2):300–305. doi: 10.1002/hep.1840160204. [DOI] [PubMed] [Google Scholar]
- Pawlotsky J. M., Fleury A., Choukroun V., Deforges L., Roudot-Thoraval F., Aumont P., Duval J., Dhumeaux D. Significance of highly positive c22-3 "indeterminate" second-generation hepatitis C virus (HCV) recombinant immunoblot assay (RIBA) and resolution by third-generation HCV RIBA. J Clin Microbiol. 1994 May;32(5):1357–1359. doi: 10.1128/jcm.32.5.1357-1359.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pistello M., Maggi F., Vatteroni L., Cecconi N., Panicucci F., Bresci G. P., Gambardella L., Taddei M., Bionda A., Tuoni M. Prevalence of hepatitis C virus genotypes in Italy. J Clin Microbiol. 1994 Jan;32(1):232–234. doi: 10.1128/jcm.32.1.232-234.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simmonds P., Alberti A., Alter H. J., Bonino F., Bradley D. W., Brechot C., Brouwer J. T., Chan S. W., Chayama K., Chen D. S. A proposed system for the nomenclature of hepatitis C viral genotypes. Hepatology. 1994 May;19(5):1321–1324. [PubMed] [Google Scholar]
- Simmonds P., Rose K. A., Graham S., Chan S. W., McOmish F., Dow B. C., Follett E. A., Yap P. L., Marsden H. Mapping of serotype-specific, immunodominant epitopes in the NS-4 region of hepatitis C virus (HCV): use of type-specific peptides to serologically differentiate infections with HCV types 1, 2, and 3. J Clin Microbiol. 1993 Jun;31(6):1493–1503. doi: 10.1128/jcm.31.6.1493-1503.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Simmonds P., Smith D. B., McOmish F., Yap P. L., Kolberg J., Urdea M. S., Holmes E. C. Identification of genotypes of hepatitis C virus by sequence comparisons in the core, E1 and NS-5 regions. J Gen Virol. 1994 May;75(Pt 5):1053–1061. doi: 10.1099/0022-1317-75-5-1053. [DOI] [PubMed] [Google Scholar]
- Stuyver L., Rossau R., Wyseur A., Duhamel M., Vanderborght B., Van Heuverswyn H., Maertens G. Typing of hepatitis C virus isolates and characterization of new subtypes using a line probe assay. J Gen Virol. 1993 Jun;74(Pt 6):1093–1102. doi: 10.1099/0022-1317-74-6-1093. [DOI] [PubMed] [Google Scholar]
- Stuyver L., van Arnhem W., Wyseur A., Hernandez F., Delaporte E., Maertens G. Classification of hepatitis C viruses based on phylogenetic analysis of the envelope 1 and nonstructural 5B regions and identification of five additional subtypes. Proc Natl Acad Sci U S A. 1994 Oct 11;91(21):10134–10138. doi: 10.1073/pnas.91.21.10134. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Doorn L. J., Kleter B., Stuyver L., Maertens G., Brouwer H., Schalm S., Heijtink R., Quint W. Analysis of hepatitis C virus genotypes by a line probe assay and correlation with antibody profiles. J Hepatol. 1994 Jul;21(1):122–129. doi: 10.1016/s0168-8278(94)80148-7. [DOI] [PubMed] [Google Scholar]