Figure 1.

Gel filtration analysis of PLD activator. (A) Purified PLD activator was loaded on a Superdex 200 column. Each fraction was assayed for PLD activation in the presence of 200 nM ARF. •, PLD activity; dotted line, A at 280 nm. Arrows indicate the elution position of ovalbumin (43 kDa) and chymotrypsinogen A (25 kDa), which were run in parallel experiments. (B) Aliquots of the samples from Superdex 200 column were subjected to SDS/PAGE on a 12.5% gel followed by silver stain. The positions of molecular mass markers are indicated in kilodaltons.