Stimulation of PLD by GM2 ganglioside activator or heat-stable PLD activator. (A) Stimulation of PLD by various amounts of purified GM2 ganglioside activator or by heat-stable PLD activator. • and ○, with GM2 ganglioside activator; ■ and □, with heat-stable PLD activator; • and ■, with 200 nM ARF; E and G, without ARF. (B) Time course of PLD reaction. •, with 56 nM GM2 ganglioside activator and 200 nM ARF; ■, with 56 nM heat-stable PLD activator and 200 nM ARF; E, with 200 nM ARF alone.