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(A) (•), |Δfsat|; (○), |Δf0|; and (×), |Δfsat| at high ionic strength (200 mM KCl) as a function of pH for HbCO. (B) (■), |Δfsat| and (□), |Δf0| as a function of pH for met-Hb. The error bars represent the SD of approximately three measurements made at each pH value. The buffers Succinic Acid, Hepes, or Tris were used depending on pH, at a concentration of 10 mM.
