Table 1.
Nuclear localization in Trypanosoma brucei
| Trypanosome clone | Markers inserted | Probe | Nucleic acid analyzed | Nuclease treatment | Overlap with nucleolus, % overlap (range) | Scoreable cells % | Cells analyzed |
|---|---|---|---|---|---|---|---|
| 221a | None | Mix 4 | RNA | DNase | 20 (18–22) | >80 | >450 |
| 3174 | neo+hyg (VSG ES) | neo+hyg | DNA | RNase | 25 (18–32) | 60 | >450 |
| RP2 | hyg (VSG ES) | hyg | DNA | RNase | 23 (20–31) | 60 | >450 |
| RPhygro | hyg (rDNA) | hgy | DNA | RNase | 75 (65–85) | 80 | >450 |
| r4 | neo (rDNA) | neo | NDA | RNase | 100 | 60 | 350 |
| 221a | None | Tubulin | DNA | RNase | 36 (32–40) | >90 | 450 |
| proCAT | cat+hyg (PARP A locus) | cat+hyg | DNA | RNase | 26 (23–30) | 60 | 450 |