FIG. 3.

MHV-68 gene expression in the absence of de novo protein synthesis. MHV-68 membrane arrays were hybridized with oligo(dT)-primed cDNA synthesized from BHK-21 cells at 8 hpi with MHV-68 at 5 PFU/cell in the absence (A) or presence (B) of CHX. (C) Fold expression of observed transcripts in treated versus untreated samples. A Storm phosphorimager and the ImageQuant system were used to quantitate the signal from the array elements corresponding to 73 known and predicted MHV-68 ORFs. GAPDH-normalized values from the +CHX array (B) were divided by the corresponding GAPDH-normalized values from the untreated (−CHX) array (A) to derive the fold inhibition of gene expression relative to the untreated level for each array element. These values and their corresponding MHV-68 ORFs are ordered in the bar graph based on increasing fold inhibition of gene expression relative to the untreated level. Statistical significance of differences in expression is assessed by paired t test (+, P > 0.05; ✽, P < 0.05; ✽✽, P < 0.01; ✽✽✽, P < 0.001). Dashed lines were placed at the 0.5-log reduction and the 1-log reduction of expression in the presence of CHX.