FIG. 3.

Distinct cell polarization and actin tails in HeLa cells infected with MVA compared to the WR strain. HeLa cells were infected with WR and MVA at 0.05 PFU/cell, and at 48 hpi cells were fixed and processed for confocal microscopy. Cytoplasmic viral p14 protein and virions were detected with an antibody to p14 protein and a secondary antibody conjugated with FITC; cellular actin was detected with phalloidin conjugated to TRITC. In cells infected with WR, individual virions (A, C, and E) and actin tails (arrow in panel C) are clearly seen in these Z-projections. The morphology of these infected cells is multipolar or stellated. Cells infected with MVA (B, D, and F) produced few virions (one is indicated by an arrow in panel D) and showed a bipolar morphology, and actin tails were not induced.