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Interaction of MLH3 and MLH1 by two-hybrid analysis. Yeast strain RDKY2926 was cotransformed with the indicated combination of one bait plasmid and one prey plasmid, and the transformants were replica plated onto Ura−His−Trp− 5-bromo-4-chloro-3-indolyl β-d-galactoside (X-Gal) glucose and Ura−His−Trp− X-Gal galactose indicator plates and incubated at 30°C for 2 days.
