FIG. 5.

γHV68 LANA is required for efficient establishment or maintenance of latency following intranasal infection. Mice were infected with 1,000 PFU of the indicated virus in 20 μl of complete DMEM. (A and C) Limiting dilution assays were performed on splenocytes plated on MEF indicator monolayers as described in Materials and Methods. (B and D) Real-time PCR analysis was performed to determine the number of viral genomes present per 0.5 μg of splenic DNA. Input DNA was standardized using a separate real-time PCR assay specific for the cellular GAPDH gene on the same DNA sample. The data were compiled from at least two independent experiments, each containing four or five mice per group. The standard error of the mean is shown.