FIG. 5.
Engagement of B cells by E1FLAGE2 complexes induces protein tyrosine phosphorylation. Human OCI-LY8 B cells were exposed to magnetic beads precoated with H53-captured E1FLAGE2 (middle), anti-CD81 MAb 5A6 (left side), or anti-E2 MAb H53 (control, right side) for the indicated times (minutes) at 37°C. The cells were lysed, and proteins containing phosphotyrosines were revealed by Western blotting with a mouse antiphosphotyrosine horseradish peroxidase-conjugated antibody 4G10. The values on the right are molecular sizes in kilodaltons.