Figure 1.

Inducible expression of murine TRAIL on DCs without induction of autocrine suicide. (a) A recombinant AdTRAIL was constructed as described in Methods. DCs from the bone marrow of DBA/1j mice were transfected with 50 pfu/cell of AdTRAIL and then incubated with different concentrations of DOX for 24 hours. The expression of functional TRAIL on the surface of the transfected cells was then evaluated by an ATPlite assay. (b) DCs from the bone marrow of DBA/1j mice were transfected with 50 pfu/cell of AdGFP, and the percentage of GFP⁺ DCs was quantitated 24 hours later under a fluorescence microscope. (c) TRAIL-sensitive HT1080 fibrosarcoma cells were transfected with 50 pfu/cell of AdTRAIL and then incubated with various concentrations of DOX for 24 hours. The expression of AdTRAIL in the target cells was tested using the ATPlite assay. Results are representative of three experiments.