TABLE 2.
SV40 J-domain mutations are defective in bacterial and bacteriophage growth
| SV40 J-domain mutation | Bacterial growtha
|
λ growthb
|
|||
|---|---|---|---|---|---|
| 14°Cc | 30°C | 40°C | λcI dnaJ+ | λcI | |
| pBAD22 (vector) | − | + | − | + | − |
| SV40 (pWKG93) | + | + | + | + | + |
| H42Q | − | + | − | + | − |
| P43F | − | + | − | + | − |
| D44N | − | + | − | + | − |
| G47E | − | + | − | + | − |
| L19F/P28S | − | + | − | + | − |
| L19F | − | + | − | + | − |
| P28S | + | + | ± | + | + |
| P43L/K45N | − | + | − | + | − |
| P43L | − | + | − | + | − |
| K45N | − | + | − | + | − |
| D44E/G47R | − | + | − | + | − |
| D44E | − | + | − | + | − |
| G47R | − | + | − | + | − |
| Δ17-27 | − | + | − | + | − |
| GmSV40 | + | + | ± | + | + |
| GmSV40 (Δ67-82) | + | + | ± | + | + |
a
Appropriate complementation for bacterial growth was obtained at 0.1% of l-arabinose inducer.
b
Appropriate complementation for bacteriophage λ growth was obtained at 0.05% of l-arabinose inducer.
c
+ indicates full complementation for bacterial and λ growth when compared to the wild-type SV40 J-domain, i.e., efficiency of approximately one in colony or plaque formation, ± indicates partial complementation (with efficiency of colony or plaque formation of <10−2), and − indicates no complementation (efficiency of colony or plaque formation of <10−5). Additional definition of efficiency is shown in the legend to Fig. 2.