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. 2003 Oct;77(19):10237–10249. doi: 10.1128/JVI.77.19.10237-10249.2003

FIG. 6.

FIG. 6.

Mapping of the PA28γ-binding region in HCV core protein. (A) 293T cells were transfected with plasmids encoding Flag-tagged HCV core deletion mutants. Endogenous PA28γ was coimmunoprecipitated with an anti-Flag antibody and was visualized by immunoblotting with anti-PA28γ antiserum. (B) Intracellular localization of EGFP-Core deletion mutants in 293T cells. All samples were observed at 24 h posttransfection. (C) EGFP-tagged HCV core deletion mutants were expressed in 293T cells. Endogenous PA28γ was coimmunoprecipitated with anti-GFP antiserum and then was visualized by immunoblotting with anti-PA28γ antiserum. IP, immunoprecipitation.