FIG. 2.

Analysis of RNA copied from 3′-mutated KUN replicon RNA by Northern blotting. Approximately 10 μg of total cell RNA was extracted from BHK cells at 45 and 69 h after transfection with 5 μg of KUN replicon RNAs mutated in the penultimate cytidine or the 3′-terminal uridine and subjected to Northern blot hybridization with ³²P-labeled cDNA probes detecting replicon RNA and β-actin mRNA as previously described (31). The mutated nucleotides are shown in boldface and underlined. Densitometry analysis of the blots is shown in Table 1.